Studies of the genetic make-up of newly discovered organisms.Performing genome-wide association studies (GWAS).Updating reference genome with newly identified mutations.Detecting large structural variants like duplications, inversions, genomic rearrangements, copy number variations (CNV).Characterising variations like SNPs and InDels.Comparing variations across multiple genomes.Re-sequencing is particularly useful for: The detailed genetic background of an individual is relevant for fields like plant and animal breeding, population genetics and clinical applications such as determining disease risk factors and discovering novel biomarkers for diseases diagnosis.Īpplications – What are the advantages of genome re-sequencing and de novo genome sequencing? This comparative sequencing enables identification of single-nucleotide polymorphisms (SNPs), insertions-deletions (InDels) and structural variations between organisms. In this process, generated reads are directly aligned to the reference sequence. In cases where a consensus sequence for a specific genome is already available, whole-genome re-sequencing can be applied. This method enables genomic characterisation of newly discovered organisms, comparative analysis of highly variable genomes and in-depth exploration of structural variants. In this process, overlapping sequencing reads are assembled into longer fragments that are further pieced together until the entire sequence of the genome is decoded.

When a genome is investigated for the first time, the process of sequencing and genome assembly is called de novo sequencing. Two types of WGS approaches are available that mainly differ in their dependency on a reference genome sequence. Whole-genome sequencing (WGS) is the sequencing of all DNA present in the genome of an organism, including chromosomal DNA, mitochondrial DNA and chloroplast DNA if in plants. Overview – What is the difference between de novo genome sequencing and genome re-sequencing?

Wg sequences